DNA Polymerases
Alkali Scientific Radiant™ Hot-Start Taq Polymerase 1000 Units 4 x 0.5mL , 5u/μL 10x PCR buffer & 50mM MgCl2
Radiant™ Hot-Start Taq Polymerase RadiantTM Hot-Start Taq DNA Polymerase is a highly purified, high performance Hot-Start DNA Polymerase optimized for the sensitive DNA amplification of a wide range of DNA templates including complex mammalian genomic DNA and crude samples. The Hot-Start technology is based on a new-generation PCR buffer formulation in conjunction with a proprietary antibody-mediated chemistry. RadiantTM Hot-Start Taq Polymerase exhibits 5´-3´ DNA polymerase activity with an error-rate of wild-type Taq ( 2.0 x 10-5). The polymerase is ideal for genotyping, colony PCR, multiplexing, low-copy assays, and the amplification of challenging targets susceptible to mispriming. RadiantTM Hot Start Taq DNA Polymerase is engineered for robust, superior PCR and is supplied with a highly optimized, new-generation 5x buffer system which provides exceptional sensitivity and ease of use. Storage RadiantTM Hot-Start Taq Polymerase is shipped on blue or dry ice and should be
New England Biolabs, Inc. Thermolabile USER II Enzyme – 50 units
Thermolabile USER (Uracil-Specific Excision Reagent) II Enzyme generates a single nucleotide gap at the location of a uracil residue. It can be 100% inactivated at temperatures >65C.
Abcam Acridine orange hemi(zinc chloride) salt, Fluorescent cationic DNA and RNA dye, 10G
MW 369.96 g/mol. Cell-permeable metachromatic fluorescent cationic dye that stains DNA and RNA. Also an inhibitor of RNA polymerase.
The product is subject to the following: Abcam Restricted Use Statement
New England Biolabs, Inc. T4 DNA Polymerase – 150 units
T4 DNA Polymerase catalyzes the synthesis of DNA in the 5' to 3' direction and requires the presence of template and primer. This enzyme has a 3' to 5' exonuclease activity which is much more active than that found in DNA Polymerase I (E. coli). Unlike E. coli DNA Polymerase I, T4 DNA Polymerase does not have a 5' to 3' exonuclease function.
Meridian Life Science MyFi DNA Polymerase, 250 units
Unique blend of highly-efficient MyTaq HS DNA Polymerase and a proprietary proofreading enzyme that combine to give increased target affinity for use with challenging templates and inhibitor-rich samples.
INTACT GENOMICS INC T4 UvsX DNA Recombinase, Lyophilized 500µg
Lyophilized T4 UvsX DNA Recombinase is useful for Recombinase Polymerase Amplification (RPA) and other applications.Homologous recombination is important for the error-free repair of DNA double-strand breaks and for replication fork restart. Recombinases of the RecA/RAD51 family perform the central catalytic role in this process. UvsX recombinase is the RecA/Rad51 ortholog of bacteriophage T4. Glycerol Free T4 UvsX DNA Recombinase is useful for Recombinase Polymerase Amplification (RPA) and other applications.Homologous recombination is important for the error-free repair of DNA double-strand breaks and for replication fork restart. Recombinases of the RecA/RAD51 family perform the central catalytic role in this process. UvsX recombinase is the RecA/Rad51 ortholog of bacteriophage T4. T4 UvsX DNA Recombinase and other recombinases form presynaptic filaments on ssDNA that are activated to search for homology in dsDNA and to perform DNA strand exchange.
As One International Inc Taq DNA Polymerase 2x Master Mix, 2.0 mM MgCl2 (final concentration), 10,000Rxn
AS ONE's Taq DNA Polymerase Pre-Mix is a ready-to-use 2.0X reaction mix. Simply add primers, template, and water to successfully carry out primer extensions and other molecular biology applications. AS ONE Taq DNA Polymerase, the NH4+ buffer system, dNTPs, and magnesium chloride are present in Taq DNA Polymerase Mix. Each reaction requires 25 uL of the 2.0X reaction mix. Simply add primers, template and water to a total reaction volume of 50 uL. Taq DNA Polymerase Pre-Mix offers several advantages. Set up time is significantly reduced. The chance of contaminating component stocks is eliminated. Reduction of reagent handling steps leads to better reproducibility. Standard tests can be set up with the confidence that results will be consistent every time.
INTACT GENOMICS INC T4 UvsX DNA Recombinase, Glycerol Free 500µg
Glycerol Free T4 UvsX DNA Recombinase is useful for Recombinase Polymerase Amplification (RPA) and other applications.Homologous recombination is important for the error-free repair of DNA double-strand breaks and for replication fork restart. Recombinases of the RecA/RAD51 family perform the central catalytic role in this process. UvsX recombinase is the RecA/Rad51 ortholog of bacteriophage T4. T4 UvsX DNA Recombinase and other recombinases form presynaptic filaments on ssDNA that are activated to search for homology in dsDNA and to perform DNA strand exchange.
As One International Inc Taq DNA Polymerase 5 U/ul 10x Standard Buffer, Magnesium free and Triton free, 2,500U
Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa. Taq DNA Polymerase has both a 5' to 3' DNA polymerase and a 5' to 3' exonuclease activity. The enzyme lacks a 3' to 5' exonuclease activity (no proofreading ability). Taq DNA Polymerase leaves an A prime overhang, which makes the enzyme ideal for TA cloning. Included with Taq is Triton/magnesium free 10x Standard Buffer.
As One International Inc Hot Start Taq Polymerase 5U/ul, 10x Combination Buffer, Magnesium free and Tween free, 2,500U
AS ONE HS Taq DNA Polymerase is a hot start polymerase designed to diminish the formation of non-specific priming events during reaction set up and the first ramp of thermal cycling. HS Taq features high specificity and sensitivity, greater yield compared to standard DNA polymerases. HS Taq enables the detection of low abundance tragets. HS Taq Polymerase includes Tween/magnesium free 10x Combination Buffer.
ABclonal Technology High Salt-T4 DNA Ligase
High Salt-T4 DNA Ligase is a salt-tolerant mutant of T4 DNA Ligase that catalyzes the formation of a phosphodiester bond between juxtaposed 5? phosphate and 3? hydroxyl termini in duplex DNA or RNA. It is designed to function at higher salt concentrations than wild type T4 DNA Ligase. The catalytic reaction requires ATP as a cofactor. High Salt-T4 DNA Ligase will join blunt end and cohesive end termini at salt concentrations as high as 300 mM without any loss in activity, and can also repair single-stranded nicks in duplex DNA, RNA or DNA/RNA hybrids. At the same time, T4 DNA ligase closes the gaps in these DNA substrates.
Sigma Aldrich Fine Chemicals Biosciences JumpStart(TM) Taq DNA Polymerase with MgCl2 | MFCD00166026 | 250UN
JumpStart(TM) Taq DNA Polymerase with MgCl2 | MFCD00166026 | 250UN
As One International Inc Red-Taq DNA Polymerase 5 U/ul 10x Ammonium Buffer, 5,000U
AS ONE Taq DNA Polymerase with red dye is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. Taq contains a red dye which provides easy and quick identification of reactions to which enzyme was added and allows confirmation of complete mixing. The inert dye has no effect on downstream processes. Taq with Red Dye is added directly to the reaction mix and is used in the same manner as standard Taq DNA Polymerase. AS ONE Taq with Red Dye DNA Polymerase has both a 5' to 3' DNA polymerase and a 5' to 3' exonuclease activity. The enzyme lacks a 3' to 5' exonuclease activity. Taq DNA Polymerase leaves an A prime overhang, which makes the enzyme ideal for TA cloning. Red Taq Polymerase includes 10x Ammonium Buffer.
As One International Inc Taq DNA Polymerase 5 U/ul glycerol free, 10x Ammonium Buffer and 10x Combination Buffer both detergent free, 1,000U
Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa. Taq DNA Polymerase has both a 5' to 3' DNA polymerase and a 5' to 3' exonuclease activity. The enzyme lacks a 3' to 5' exonuclease activity (no proofreading ability). Taq DNA Polymerase leaves an A prime overhang, which makes the enzyme ideal for TA cloning. Glycerol free Taq is ideal for automation and free drying applications. Included with Taq are Tween/Triton free 10x Ammonium Buffer and 10x Combination Buffer.